Gas Chromatography :: essays research papers

GasChromatographyPurpose The purpose of the triggerman chromatography research science research laboratoryoratoryoratory is to find out how contrastive substances interact with the surface of a square(a). Chromatography is a separation technique that dep force outs on the relative distribution of the components of a florilegium between a mobile phase and a inviolable unmoving phase. Chromatography measures the tendency of a substance to interact with the surface of a square(a) or to remain in a mobile phase. When doing a chromatography lab the mobile phase has to be a substance that is either in a liquid or a torpedo state. In this lab the mobile phase was a gun, which is why this is called a gas chromatography lab. The different gases tested in this lab were CHCl3 and CH2Cl2. It is determined to what extent a gas interacts with the solid by injecting a known amount of the mobile gas into the carrier gas and then measuring the concentration that comes out at the end of the tower. From this there was a detector that transferred the information to a reckoner were it was graphed. The tendency of the gas to interact with the solid is determined by the itemize of theoretical plates. A substance that interacts more strongly with the surface of the solid will take more time to be carried across the stationary phase.ProcedureThe pieces of a Gas Chromatograph are the gas supply, injector, column and the detector. The gas supply, or carrier gas, is the gas from the valves at the lab tables. First a coil had to be made out of grunter, which would serve as the burner for the maculation system. A pipette was used as the column to put the solid stationary substance into. The solid phase in this experiment was Tide. The pipet was filled with Tide detergent and cotton was inserted in both end of the pipet. The column was then secured horizontally to a ring stand development clamps. The tip of the column should be in a vertical position. The copper coil is then placed in the vertical part of the column with the coil about 1/8 above the end of the column. It is grievous that the copper coil be placed at the right top side because if it is too low the flame will not get large air and if the copper is to high the flame will burn below the coil.